anti-PCK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; PCK1; anti-PCK1 antibody; PEPCK1;
The PCK1 pck1 (Catalog #MBS6008048) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The PEPCK1 (Phosphoenolpyruvate Carboxykinase 1, PEPCK-1, Pck1, Phosphoenolpyruvate Carboxykinase Cytosolic [GTP], PEP Carboxykinase, PEPCK, PEPCKC, PEPCK-C, Phosphoenolpyruvate Carboxylase, HGNC:8724, MGC22652) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PEPCK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:16,000
Western Blot: 0.5-1.5ug/ml, observed in human kidney lysates on ~70kD bands. Researchers should empirically determine the suitability of the PCK1 pck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PCK1 pck1 product has the following accession number(s) (GI #187281517) (NCBI Accession #NP_002582.3) (Uniprot Accession #P35558). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PEPCK1 (Phosphoenolpyruvate Carboxykinase 1, PEPCK-1, Pck1, Phosphoenolpyruvate Carboxykinase Cytosolic [GTP], PEP Carboxykinase, PEPCK, PEPCKC, PEPCK-C, Phosphoenolpyruvate Carboxylase, HGNC:8724, MGC22652) with the following immunoassay(s):
Chemiluminescence ((0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Immunogen: Synthetic peptide corresponding to C-EKEVEDIEKYLEDQ, from human PCK1, at the internal region (near C-terminal) of the protein (NP_002582.2). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PCK1 are readily searchable from our website. Different antibodies against the same target such as PCK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Diabetes Mellitus, Diabetes Mellitus, Type 2, Fatty Liver, Glucose Intolerance, Hyperglycemia, Inflammation, Insulin Resistance, Liver Diseases, Obesity, Weight Gain are some of the diseases may be linked to PEPCK1 (Phosphoenolpyruvate Carboxykinase 1, PEPCK-1, Pck1, Phosphoenolpyruvate Carboxykinase Cytosolic [GTP], PEP Carboxykinase, PEPCK, PEPCKC, PEPCK-C, Phosphoenolpyruvate Carboxylase, HGNC:8724, MGC22652). The following patways have been known to be associated with this gene.