anti-PCSK9 antibody product blog
Tags: Antibody; PCSK9; Polyclonal Antibody; anti-PCSK9 antibody;
The PCSK9 pcsk9 (Catalog #MBS176261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCSK9 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PCSK9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the PCSK9 pcsk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The PCSK9 pcsk9 product has the following accession number(s) (GI #317373487) (NCBI Accession #Q8NBP7.3) (Uniprot Accession #Q8NBP7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PCSK9 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-PCSK9 antibody, MBS176261, Western blotting
All lanes: Anti PCSK9 (MBS176261) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Predicted bind size: 74KD
Observed bind size: 74KD)
Immunohistochemistry (IHC) (Anti-PCSK9 antibody, MBS176261, IHC(P)
IHC(P): Human Intestinal Cancer Tissue )
Description: Rabbit IgG polyclonal antibody for Proprotein convertase subtilisin/kexin type 9(PCSK9) detection. Tested with WB, IHC-P in Human.
Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. PCSK9 can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PCSK9 (671-687aa AVTAVAICCRSRHLAQA). Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PCSK9 are readily searchable from our website. Different antibodies against the same target such as PCSK9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.