|The PDE11A pde11a (Catalog #MBS242151) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human PDE11A reacts with Bat, Gibbon, Bovine, Dog, Gorilla, Human, Monkey, Pig
Predicted Reactivity: Mouse, Rat, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s PDE11A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:2000), IHC-P (10 ug/ml), WB (0.5 - 1.5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PDE11A pde11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDE11A pde11a product has the following accession number(s) (GI #116536083) (NCBI Accession #NP_001070664.1) (Uniprot Accession #Q9HCR9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human PDE11A with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-PDE11A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB) (Antibody (1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Phosphodiesterase 11A is a cAMP/cGMP dual-specific phosphodiesterase. PDE11A activity is inhibited by IBMX, as well as zaprinast and dipyridamole. At least four isoforms have been identified which carry distinct GAF sequences in the N-terminal regions. PDE11A2 contains an additional 86 amino acids at the N-terminus. PDE11A3 contains an additional 108 N-terminal amino acids compared to PDE11A2. PDE11A4 is the longest isoform.
Target Species: Human
Immunogen Description: Synthetic peptide C-NSFKESMEKSSYSD from an internal region of human PDE11A (NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1). Immunogen Type: Synthetic peptide
Immunogen: PDE11A antibody was raised against synthetic peptide C-NSFKESMEKSSYSD from an internal region of human PDE11A (NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Pig (100%); Mouse, Rat, Hamster, Horse, Rabbit, Opossum (93%); Xenopus, Stickleback, Pufferfish, Zebrafish (86%).
Antigen Modification: Internal. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDE11A are readily searchable from our website. Different antibodies against the same target such as PDE11A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.