anti-PDGFR alpha antibody product blog
Tags: Antibody; Polyclonal Antibody; PDGFR alpha; anti-PDGFR alpha antibody;
The PDGFR alpha pdgfra (Catalog #MBS9408573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDGFR alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PDGFR alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the PDGFR alpha pdgfra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDGFR alpha pdgfra product has the following accession number(s) (GI #5453870) (NCBI Accession #NP_006197.1) (Uniprot Accession #P16234). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PDGFR alpha Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human brain tissue using PDGFR an antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using PDGFR an antibody.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using PDGFR an antibody.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells (Lane 2), using PDGFR an antiobdy. The lane on the left is treated with synthesized peptide.)
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.Jennifer Brennan, Genes & Dev., Mar 2003; 17: 800.JC Yu, J. Biol. Chem., Apr 1994; 269: 10668 - 10674.Nobuyuki Takakura, J. Histochem. Cytochem., Jun 1997; 45: 883.