anti-PDHA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-PDHA1 antibody; PDHA1; BDHA1;
The PDHA1 pdha1 (Catalog #MBS9205911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BDHA1 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s BDHA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS).WB~~1:1000. Researchers should empirically determine the suitability of the PDHA1 pdha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDHA1 pdha1 product has the following accession number(s) (GI #4505685) (NCBI Accession #NP_000275.1) (Uniprot Accession #P08559). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BDHA1 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (BDHA1 Antibody (Center) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the BDHA1 antibody detected the BDHA1 protein (arrow).)
Flow Cytometry (FC/FACS) (BDHA1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
The pyruvate dehydrogenase (PDH) complex is a
nuclear-encoded mitochondrial multienzyme complex that catalyzes
the overall conversion of pyruvate to acetyl-CoA and CO(2), and
provides the primary link between glycolysis and the tricarboxylic
acid (TCA) cycle. The PDH complex is composed of multiple copies of
three enzymatic components: pyruvate dehydrogenase (E1),
dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase
(E3). The E1 enzyme is a heterotetramer of two alpha and two beta
subunits. This gene encodes the E1 alpha 1 subunit containing the
E1 active site, and plays a key role in the function of the PDH
complex.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDHA1 are readily searchable from our website. Different antibodies against the same target such as PDHA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Connective Tissue, Embryonic Tissue, Eye, Liver, Lung, Muscle, Skin, Testis tissues are correlated with this protein. PDHA1 also interacts with the following gene(s): DLAT, DLD, PDHB, PDHX, PDK1, PDK2, PDK3, PDK4.