|The PDIA6 pdia6 (Catalog #MBS9211253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDIA6 Antibody (Center D251) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s PDIA6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).
WB~~1:1000. Researchers should empirically determine the suitability of the PDIA6 pdia6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDIA6 pdia6 product has the following accession number(s) (GI #5031973) (NCBI Accession #NP_005733.1) (Uniprot Accession #Q15084). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PDIA6 Antibody (Center D251) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of PDIA6 Antibody (Center D251) in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane). PDIA6 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center D251), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of hela cells using PDIA6 Antibody (Center D251)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Protein disulfide isomerases (EC 22.214.171.124), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDIA6 are readily searchable from our website. Different antibodies against the same target such as PDIA6 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Intestine, Kidney, Liver, Lung, Lymph Node, Muscle, Pancreas, Vascular tissues are correlated with this protein. PDIA6 also interacts with the following gene(s): HSP90B1, HSPA5, P4HB, UBC.