anti-PDPK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-PDPK1 antibody; PDPK1; Phospho-PDPK1 (S396);
The PDPK1 pdpk1 (Catalog #MBS9214216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PDPK1 (S396) Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Phospho-PDPK1 (S396) can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA).DB~~1:500. Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDPK1 pdpk1 product has the following accession number(s) (GI #387849239) (NCBI Accession #NP_001248745.1) (Uniprot Accession #O15530). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Phospho-PDPK1 (S396) Antibody with the following immunoassay(s):
Dot Blot (DB) (Dot blot analysis of anti-PDPK1-pS396 Phospho-specific Pab (RB13093) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDPK1 are readily searchable from our website. Different antibodies against the same target such as PDPK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Heart, Kidney, Liver, Lung, Muscle, Ovary, Prostate, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. PDPK1 also interacts with the following gene(s): AKT1, HSP90AA1, PDK1, PRKCE, PRKCZ, RPS6KA3, RPS6KB1, SGK1, STRAP. Adenocarcinoma, Cardiovascular Diseases, Cell Transformation, Neoplastic, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Myocardial Infarction, Necrosis are some of the diseases may be linked to Phospho-PDPK1 (S396) Antibody.