anti-PDX1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Pdx1; anti-PDX1 antibody;
The PDX1 pdx1 (Catalog #MBS624035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDX1 (T11) (Pyruvate Dehydrogenase Protein X Component, Mitochondrial, Dihydrolipoamide Dehydrogenase-binding Protein of Pyruvate Dehydrogenase Complex, Lipoyl-containing Pyruvate Dehydrogenase Complex Component X, E3-binding Protein, E3BP, PDHX) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PDX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the PDX1 pdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDX1 pdx1 product has the following accession number(s) (GI #4557673) (NCBI Accession #NP_000200.1) (Uniprot Accession #P52945). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PDX1 (T11) (Pyruvate Dehydrogenase Protein X Component, Mitochondrial, Dihydrolipoamide Dehydrogenase-binding Protein of Pyruvate Dehydrogenase Complex, Lipoyl-containing Pyruvate Dehydrogenase Complex Component X, E3-binding Protein, E3BP, PDHX) with the following immunoassay(s):
Immunofluorescence (IF) (Fluorescent confocal image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2034d PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data.)
Western Blot (WB) (Western blot analysis of anti-PDX1 Antibody (T11) in NCI-H460 cell line lysates (35ug/lane).PDX1-T11(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.