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anti-PECAM1 antibody product blog

Posted on 2015-08-03 15:40:43 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CD31; anti-PECAM1 antibody; PECAM1;
The PECAM1 pecam1 (Catalog #MBS176169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD31 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin. Researchers should empirically determine the suitability of the PECAM1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PECAM1 pecam1 product has the following accession number(s) (GI #129747) (NCBI Accession #P16284.1) (Uniprot Accession #P16284). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-CD31 antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CD31 antibody, MBS176169, IHC(P)
IHC(P): Human Intestinal Cancer Tissue )
Immunohistochemistry (IHC) PECAM1.

Immunohistochemistry (IHC) (Anti-CD31 antibody, MBS176169, IHC(P)
IHC(P): Human Placenta Tissue )
Immunohistochemistry (IHC) PECAM1.

Immunohistochemistry (IHC) (Anti-CD31 antibody, MBS176169, IHC(F)
IHC(F): Human Placenta Tissue )
Immunohistochemistry (IHC) PECAM1.

Western Blot (WB) (Anti-CD31 antibody, MBS176169,Western blotting
All lanes: Anti CD31 (MBS176169) at 0.5ug/ml
WB : Human Placenta Tissue Lysate at 50ug
Predicted bind size: 82 KD
Observed bind size: 82KD)
Western Blot (WB) PECAM1.

Description: Rabbit IgG polyclonal antibody for Platelet endothelial cell adhesion molecule(PECAM1) detection. Tested with WB, IHC-P, IHC-F in Human.
Background: PECAM-1(Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31(CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin(Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al.(1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller(1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD31 (717-732aa RKAVPDAVESRYSRTE). Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PECAM1 are readily searchable from our website. Different antibodies against the same target such as PECAM1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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