anti-PGK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; PGK1; anti-PGK1 antibody;
The PGK1 pgk1 (Catalog #MBS176104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGK1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PGK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PGK1 pgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The PGK1 pgk1 product has the following accession number(s) (GI #52788229) (NCBI Accession #P00558.3) (Uniprot Accession #P00558). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PGK1 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-PGK1 antibody, MBS176104, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: A431 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: A549 Cell Lysate
Lane 8: JURKAT Cell Lysate )
Immunohistochemistry (IHC) (Anti-PGK1 antibody, MBS176104, IHC(P)
IHC(P): Human Lung Cancer Tissue )
Description: Rabbit IgG polyclonal antibody for Phosphoglycerate kinase 1(PGK1) detection. Tested with WB in Human, Mouse, Rat.
Background: PGK1(Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al.(1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al.(2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.
Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PGK1 (166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PGK1 are readily searchable from our website. Different antibodies against the same target such as PGK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.