anti-PKM antibody product blog
Tags: Antibody; PKM2; Polyclonal Antibody; anti-PKM antibody; PKM;
The PKM pkm (Catalog #MBS9214301) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKM2 (N-term E131) Antibody reacts with Human (Predicted Reactivity: Mouse, Rabbit, Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s PKM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000. Researchers should empirically determine the suitability of the PKM pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PKM pkm product has the following accession number(s) (GI #332164775) (NCBI Accession #NP_001193725.1) (Uniprot Accession #P14618). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PKM2 (N-term E131) Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).)
Immunohistochemistry (IHC) (PKM2 (N-term E131) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (PKM2 (N-term E131) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PKM are readily searchable from our website. Different antibodies against the same target such as PKM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.