anti-PLA2G2A antibody product blog
Tags: Antibody; Polyclonal Antibody; PLA2G2A; anti-PLA2G2A antibody;
The PLA2G2A pla2g2a (Catalog #MBS619587) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The PLA2G2A (phospholipase A2, group IIA (platelets, synovial fluid)GIIC sPLA2, Group IIA phospholipase A2, MOM1, Non-pancreatic secretory phospholipase A2, NPS-PLA2, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2, membrane associated precursor, PLA2, reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PLA2G2A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP).Suitable for use in Direct ELISA, Immunoprecipitation and Western Blot.
Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human PLA2G2A is ~1.5ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human PLA2G2A is ~2ng/lane under non-reducing conditions and ~50ng/lane under reducing conditions.
Immunoprecipitation: Used to immunoprecipitate recombinant human PLA2G2A from conditioned media of transfected Sf21 cells. Researchers should empirically determine the suitability of the PLA2G2A pla2g2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PLA2G2A pla2g2a product has the following accession number(s) (GI #239915991) (NCBI Accession #NP_001155201.1) (Uniprot Accession #P14555). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Phospholipases A2 (PLA2s) hydrolyze the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids. They play an important role in a variety of cellular processes, including digestion and metabolism of phospholipids, as well as production of precursors for inflammatory reactions. Phospholipases A2 belongs to PLA2 group II, which consists of extracellular enzymes with low molecular masses that require calcium ions for catalysis.
Immunogen: Recombinant protein corresponding to aa21-144 of human PLA2G2A expressed in Sf21 cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PLA2G2A are readily searchable from our website. Different antibodies against the same target such as PLA2G2A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.