anti-PLK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; PLK1; anti-PLK1 antibody;
The PLK1 n/a (Catalog #MBS621760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Plk1, phosphorylated (Thr210) (STPK13, Polo-like kinase 1, Serine-threonine kinase 13) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Plk1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence Microscopy.
Dilution: ELISA: 1:3000-1:12,000
Western Blot: 1:200-1:2000 (~68kD)
Immunohistochemistry: 1:200-1:1000 on kidney, liver, cancer, thyriod, and lymphocyte tissue. Researchers should empirically determine the suitability of the PLK1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PLK1 n/a product has the following accession number(s) (GI #548520203) (NCBI Accession #XP_005697666.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk1 occurs on Thr210 for activation. Plk1 is involved in the mitotic spindle and mitosis and is associated with the phosphorylation of cyclin B1. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
Immunogen: Synthetic peptide corresponding to aa 205-214 of Human Polo Kinase I (Plk1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PLK1 are readily searchable from our website. Different antibodies against the same target such as PLK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.