anti-PML antibody product blog
Tags: Antibody; Polyclonal Antibody; PML; anti-PML antibody;
The PML pml (Catalog #MBS9405423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PML Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PML can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the PML pml for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PML pml product has the following accession number(s) (GI #4505903) (NCBI Accession #NP_002666.1) (Uniprot Accession #P29590). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PML Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of HeLa cell lysate using PML antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human endometrial cancer using PML antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human thyroid cancer using PML antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse kidney using PML antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using PML antibody. Blue: DAPI for nuclear staining.)
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein\'s central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen Type: Recombinant Protein
Immunogen Description: Recombinant protein of human PML. Target Name: PML. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PML are readily searchable from our website. Different antibodies against the same target such as PML may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.