anti-pofut1 antibody product blog
Tags: Antibody; Polyclonal Antibody; POFUT1; anti-POFUT1 antibody; protein O-fucosyltransferase 1;
The pofut1 n/a (Catalog #MBS620923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Protein O-Fucosyltransferase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 0.5-1ug/ml. Researchers should empirically determine the suitability of the pofut1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The pofut1 n/a product has the following accession number(s) (GI #157278551) (NCBI Accession #NP_001098376.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Protein O-Fucosyltransferase 1 (POFUT1, FUT12, GDP-Fucose Protein O-Fucosyltransferase 1 Precursor, KIAA0180, MGC2482, O-Fuc-T, O-FucT-1, O-FUT, Peptide-O-fucosyltransferase, Peptide-O-fucosyltransferase 1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.)
POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Immunogen: Synthetic peptide corresponding to 16aa from near the amino terminus of human POFUT1. Positive Control: Human Heart Tissue Lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing pofut1 are readily searchable from our website. Different antibodies against the same target such as pofut1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.