anti-POLG antibody product blog
Tags: Antibody; Polyclonal Antibody; Polg; DNA polymerase gamma; anti-POLG antibody;
The POLG polg (Catalog #MBS9406234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA Polymerase gamma antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNA Polymerase gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the POLG polg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The POLG polg product has the following accession number(s) (GI #4505937) (NCBI Accession #NP_002684). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DNA Polymerase gamma antibody with the following immunoassay(s):
Testing Data (Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE Primary antibody diluted at 1: 1000)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Skeletal Muscle, using DNA polymerase gamma antibody(10 ug/ml).)
Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody at 1: 200 dilution.)
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Immunogen Type: Recombinant protein
Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 779 and 1191 (P54098) of DNA polymerase gamma. Target Name: DNA Polymerase gamma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing POLG are readily searchable from our website. Different antibodies against the same target such as POLG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. POLG also interacts with the following gene(s): C10orf2, DGUOK, MPV17, POLD1, POLG2, SLC25A4, TFAM, TK2, TYMP.