anti-POLG2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-POLG2 antibody; POLG2;
The POLG2 polg2 (Catalog #MBS9125622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLG2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s POLG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the POLG2 polg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The POLG2 polg2 product has the following accession number(s) (GI #17367139) (NCBI Accession #Q9UHN1.1) (Uniprot Accession #Q9UHN1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the POLG2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using POLG2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat heart using POLG2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using POLG2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse testis using POLG2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using POLG2 antibody at dilution of 1:100 (40x lens).)
This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human POLG2
Calculated Molecular Weight: 54kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing POLG2 are readily searchable from our website. Different antibodies against the same target such as POLG2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.