anti-PPIF antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-PPIF antibody; PPIF;
The PPIF ppif (Catalog #MBS126954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIF Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s PPIF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:50. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PPIF ppif product has the following accession number(s) (GI #231968) (NCBI Accession #P30405.1) (Uniprot Accession #P30405). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PPIF Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PPIF antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.)
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008].
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human PPIF
Calculated Molecular Weight: 22kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PPIF are readily searchable from our website. Different antibodies against the same target such as PPIF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Embryonic Tissue, Heart, Intestine, Kidney, Liver, Lung, Muscle, Pancreas tissues are correlated with this protein. Cardiovascular Diseases, Disease Models, Animal, Drug-Induced Liver Injury, Drug-Related Side Effects and Adverse Reactions, Fibrosis, Hypertrophy, Liver Diseases, Necrosis, Nervous System Diseases, Poisoning are some of the diseases may be linked to PPIF Polyclonal Antibody.