anti-PRAME antibody product blog
Tags: Antibody; Polyclonal Antibody; PRAME; anti-PRAME antibody;
The PRAME prame (Catalog #MBS627474) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAME, CT (Preferentially Expressed Antigen of Melanoma, Melanoma Antigen Preferentially Expressed in Tumors, MAPE, CT130, OPA Interacting Protein 4, OPA-interacting Protein 4, Opa-interacting Protein OIP4, OIP4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PRAME can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the PRAME prame for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRAME prame product has the following accession number(s) (GI #47678629) (Uniprot Accession #P78395). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRAME, CT (Preferentially Expressed Antigen of Melanoma, Melanoma Antigen Preferentially Expressed in Tumors, MAPE, CT130, OPA Interacting Protein 4, OPA-interacting Protein 4, Opa-interacting Protein OIP4, OIP4) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
Immunogen: Synthetic peptide selected from the C-terminal region of human MAPE (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRAME are readily searchable from our website. Different antibodies against the same target such as PRAME may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.