anti-PRAME antibody product blog
Tags: Antibody; Polyclonal Antibody; PRAME; anti-PRAME antibody;
The PRAME prame (Catalog #MBS645405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAME (Melanoma Antigen Preferentially Expressed in Tumors, Opa-interacting Protein 4, OIP-4, Preferentially Expressed Antigen of Melanoma, MAPE, OIP4) reacts with Canine, Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s PRAME can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000-1:5000. Researchers should empirically determine the suitability of the PRAME prame for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRAME prame product has the following accession number(s) (GI #47678629) (NCBI Accession #CAG30435.1) (Uniprot Accession #P78395). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRAME (Melanoma Antigen Preferentially Expressed in Tumors, Opa-interacting Protein 4, OIP-4, Preferentially Expressed Antigen of Melanoma, MAPE, OIP4) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows MBS645405 tested at 1:5000 dilution.)
PRAME is a 509aa protein belonging to the PRAME family consisting of 7 LRR (leucine-rich) repeats. It is a tumor antigen expressed in human melanomas and is recognized by cytolytic T lymphocytes. It\'s overexpression frequently observed in human cancers confers growth or survival advantages by antagonizing retinoic acid receptor (RAR) signaling. PRAME serves as a candidate marker for monitoring childhood acute leukemia. It is expressed in testis and acute leukemias.
Immunogen: Synthetic peptide corresponding to aa460-510 of human PRAME. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRAME are readily searchable from our website. Different antibodies against the same target such as PRAME may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. PRAME also interacts with the following gene(s): CUL2. Blood, Bone Marrow, Brain, Connective Tissue, Eye, Kidney, Lung, Lymph, Skin, Testis tissues are correlated with this protein. Breast Neoplasms, Carcinoma, Renal Cell, Leukemia, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Lung Diseases, Melanoma, Multiple Myeloma, Neoplasms, Ovarian Neoplasms, Sarcoma are some of the diseases may be linked to PRAME (Melanoma Antigen Preferentially Expressed in Tumors, Opa-interacting Protein 4, OIP-4, Preferentially Expressed Antigen of Melanoma, MAPE, OIP4).