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anti-PRG2 antibody product blog

Posted on 2017-10-08 09:47:20 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-PRG2 antibody; PRG2; PRG2 / Proteoglycan 2;
The PRG2 prg2 (Catalog #MBS249802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRG2 / Proteoglycan 2 Antibody IHC-plus reacts with Mouse, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PRG2 / Proteoglycan 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB).
IHC (1:50 - 1:200)
IHC - Paraffin (1:200)
Western blot (1:500 - 1:2000)
(optimal dilution to be determined by the researcher). Researchers should empirically determine the suitability of the PRG2 prg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PRG2 prg2 product has the following accession number(s) (GI #46276889) (NCBI Accession #NP_002719.3) (Uniprot Accession #P13727). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PRG2 / Proteoglycan 2 Antibody IHC-plus with the following immunoassay(s):
Immunohistochemistry - Paraffin (IHC) (Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC) PRG2.

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRG2 antibody.)
Western Blot (WB) PRG2.

PRG2 Antibody, BMPG Antibody, Bone marrow proteoglycan Antibody, Bone-marrow proteoglycan Antibody, Eosinophil major basic protein Antibody, MBP1 Antibody, Natural killer cell activator Antibody, Proteoglycan 2 Antibody, Proteoglycan 2 preproprotein Antibody Description: PRG2 / Proteoglycan 2 is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions.

Target: PRG2/Proteoglycan 2
Immunogen Species: PRG2 / Proteoglycan 2 antibody was raised against Human
Immunogen: PRG2 / Proteoglycan 2 antibody was raised against recombinant human PRG2.
Immunogen Description: Recombinant human PRG2.
Antigen Type: Recombinant protein. Target Species: Human
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

(Do not allow tissues to dry at any time during the staining procedure)
Apply an universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip.
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