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anti-PRKAB1 antibody product blog

Posted on 2015-08-26 14:50:48 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; PRKAB1; AMPK beta (PRKAB1); anti-PRKAB1 antibody;
The PRKAB1 prkab1 (Catalog #MBS9209819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMPK beta (PRKAB1) Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s AMPK beta (PRKAB1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC).
WB~~1:1000. Researchers should empirically determine the suitability of the PRKAB1 prkab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PRKAB1 prkab1 product has the following accession number(s) (GI #19923359) (NCBI Accession #NP_006244.2) (Uniprot Accession #Q9Y478). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AMPK beta (PRKAB1) Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (The anti-PRKAB1 Pab is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).)
Western Blot (WB) PRKAB1.

Western Blot (WB) (Western blot analysis of anti-PRKAB1 Antibody (N-term) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.)
Western Blot (WB) PRKAB1.

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC) PRKAB1.

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRKAB1 are readily searchable from our website. Different antibodies against the same target such as PRKAB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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