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anti-Prkab2 antibody product blog

Posted on 2016-01-02 01:18:08 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-Prkab2 antibody; Prkab2; AMP Activated Protein Kinase beta1;
The Prkab2 prkab2 (Catalog #MBS627924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMP Activated Protein Kinase beta1, phosphorylated (Ser182) (5\'-AMP-activated Protein Kinase Subunit beta-1, AMPK Subunit beta-1, AMPKb, PRKAB1, AMPK) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s AMP Activated Protein Kinase beta1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:4,000
Western Blot: 1:500-1:1,000
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the Prkab2 prkab2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Prkab2 prkab2 product has the following accession number(s) (GI #6013219) (NCBI Accession #AAF01293.1) (Uniprot Accession #Q9QZH4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK is also involved in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP inhibits the dephosphorylation and inactivation of AMPK by PP2C. The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation. Phosphorylation at serine 108 of the beta1 subunit seems to be required for the activation of AMPK enzyme. Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization.

Prkab2 prkab2

Immunogen: Synthetic phosphopeptide corresponding to human AMPK beta1 around the phosphorylation site of serine 182 (S-S-SP-P-P). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Prkab2 are readily searchable from our website. Different antibodies against the same target such as Prkab2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Heart tissues are correlated with this protein.
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