anti-Prkcg antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-PRKCG antibody; PRKCG;
The Prkcg prkcg (Catalog #MBS624568) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKCG, CT (Protein Kinase C, gamma type, PKC-gamma, PKCG) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s PRKCG can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the Prkcg prkcg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Prkcg prkcg product has the following accession number(s) (GI #6981400) (NCBI Accession #NP_036760.1) (Uniprot Accession #P63319). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRKCG, CT (Protein Kinase C, gamma type, PKC-gamma, PKCG) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis in mouse brain lysate using MBS624568. PKC gamma (arrow) was detected using MBS624568. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue using MBS624568, and peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS624568 for immunohistochemistry.)
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
Immunogen: Synthetic peptide selected from the C-terminal region of human PKC (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Prkcg are readily searchable from our website. Different antibodies against the same target such as Prkcg may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.