anti-PRKCZ antibody product blog
Tags: Antibody; Polyclonal Antibody; PKC Zeta; anti-PRKCZ antibody; PRKCZ;
The PRKCZ prkcz (Catalog #MBS352244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-human Protein Kinase C zeta [480-492] Serum reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PKC Zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot.Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:250 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the PRKCZ prkcz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRKCZ prkcz product has the following accession number(s) (GI #75709228) (NCBI Accession #NP_001028754.1) (Uniprot Accession #Q05513). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The antiserum was raised in a rabbit which was immunized with synthetic Protein Kinase C zeta [480-492] (rat) covalently attached onto a carrier protein. Rehydrate the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum. This antiserum has been found to stain specifically fixed cultured cells by indirect immunofluorescence. The antiserum was tested for recognition of other Protein Kinase C isozymes by ELISA techniques.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRKCZ are readily searchable from our website. Different antibodies against the same target such as PRKCZ may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.