|The PRKCZ prkcz (Catalog #MBS9209322) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKC zeta Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PKC zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC).
WB~~1:1000. Researchers should empirically determine the suitability of the PRKCZ prkcz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRKCZ prkcz product has the following accession number(s) (GI #75709226) (NCBI Accession #NP_001028753.1) (Uniprot Accession #Q05513). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PKC zeta Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of anti-PKCzeta Pab in placenta lysate. PKCzeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKC zeta antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a constitutive kinase activity which is independent of calcium, and PKC activators, phosphatidylserine and diacylglycerol. Furthermore, it is insensitive to PKC inhibitors and cannot be activated by phorbol ester. The structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRKCZ are readily searchable from our website. Different antibodies against the same target such as PRKCZ may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Eye, Heart, Kidney, Liver, Lung, Lymph, Ovary, Prostate, Uterus tissues are correlated with this protein.