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anti-PRMT5 antibody product blog

Posted on 2015-12-17 09:45:12 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; PRMT5; anti-PRMT5 antibody;
The PRMT5 prmt5 (Catalog #MBS9405987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRMT5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PRMT5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (CHIP).
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunoprecipitation: 1:20 - 1:100
Chromatin immunoprecipitation: 1:20 - 1:100. Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PRMT5 prmt5 product has the following accession number(s) (GI #88900507) (NCBI Accession #NP_001034708.1) (Uniprot Accession #O14744). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PRMT5 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRMT5 antibody.)
Western Blot (WB) PRMT5.

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using PRMT5 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) PRMT5.

Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.

Immunogen Type: Peptide
Immunogen Description: A synthetic peptide of human PRMT5. Target Name: PRMT5. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRMT5 are readily searchable from our website. Different antibodies against the same target such as PRMT5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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