anti-PROX1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Prox1; anti-PROX1 antibody;
The PROX1 prox1 (Catalog #MBS624248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PROX1, ID (Prospero Homeobox Protein 1, Homeobox Prospero-like Protein PROX1, PROX-1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PROX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PROX1 prox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PROX1 prox1 product has the following accession number(s) (GI #397174762) (NCBI Accession #NP_001257545.1) (Uniprot Accession #Q92786). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PROX1, ID (Prospero Homeobox Protein 1, Homeobox Prospero-like Protein PROX1, PROX-1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of anti-PROX1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). PROX1(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PROX1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. Prox1 is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been documented to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.