anti-PSMD5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-PSMD5 antibody; PSMD5; Proteasome 26S Subunit;
The PSMD5 psmd5 (Catalog #MBS2026758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 5 (PSMD5) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Proteasome 26S Subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).Western blotting: 1-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PSMD5 psmd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PSMD5 psmd5 product has the following accession number(s) (GI #394025672) (NCBI Accession #NP_001257356.1) (Uniprot Accession #Q16401). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KAAIKSLS RISLTQAGLE ALFESNLLDD LKSVMKTNDI VRYRVYELII EISSVSPESL NYCTTSGLVT QLLRELTGED VLVRATCIEM VTSLAYTHHG RQYLAQEGVI DQISNIIVGA DSDPFSSFYL PGFVKFFGNL AVMDSPQQIC ERYPIFVEKV FEMIESQDPT MIGVAVDTVG ILGSNVEGKQ VLQKTGTRFE R.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Proteasome 26S Subunit, Non ATPase 5 (PSMD5) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Human Jurkat Cells.)
Western Blot (WB) (Western Blot: Sample: Recombinant PSMD5, Human.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Knockout Validation (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: PSMD5 knockout Jurkat cell lysate; Predicted MW: 51,56kd Observed MW: 50kd Primary Ab: 3ug/ml Rabbit Anti-Human PSMD5 Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Fragment: PSMD5 (Lys143~Arg341)
Organism Species: Homo sapiens (Human)
Cross Reactivity: Human. Conjugate: No Conjugate
Immunogen: Recombinant PSMD5 (Lys143~Arg341) expressed in E Coli.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2077720. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PSMD5 are readily searchable from our website. Different antibodies against the same target such as PSMD5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.