anti-PTN antibody product blog
Tags: Antibody; PTN; Polyclonal Antibody; anti-PTN antibody;
The PTN ptn (Catalog #MBS6012909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTN, CT (PTN, HBNF1, NEGF1, Pleiotrophin, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor 1, Osteoblast-specific factor 1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PTN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the PTN ptn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PTN ptn product has the following accession number(s) (GI #4506281) (NCBI Accession #NP_002816.1) (Uniprot Accession #P21246). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PTN, CT (PTN, HBNF1, NEGF1, Pleiotrophin, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor 1, Osteoblast-specific factor 1) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis in U251,NCI-H460 cell line lysates (35ug/lane) using MBS6012909. This demonstrates that MBS6012909 detected the PTN protein (arrow).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6012909 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6012909 for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of U251 cells (right histogram) using MBS6012909compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PTN ia a heparin binding mitogenic protein. It has neurite extension activity.
Immunogen: PTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the C-terminal region of human PTN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PTN are readily searchable from our website. Different antibodies against the same target such as PTN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.