anti-RAD9 antibody product blog
Tags: Antibody; Polyclonal Antibody; RAD9; anti-RAD9 antibody;
The RAD9 f22f7.7 (Catalog #MBS616243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAD9, phosphorylated (Ser1129) reacts with Yeast and may cross-react with other species as described in the data sheet. MyBioSource\'s RAD9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA).Suitable for use in ELISA.
Dilution: ELISA: 1:5000. 0.1ug of phosphorylated peptide (pS1129) was used in a standard capture ELISA using TMB (3,3\',5,5\'-Tetramethylbenizidine) as a substrate for 30 minutes at RT. Researchers should empirically determine the suitability of the RAD9 f22f7.7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAD9 f22f7.7 product has the following accession number(s) (GI #79313129) (NCBI Accession #NP_001030644.1) (Uniprot Accession #Q9MA61). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9. Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions. Another cellular strategy is to delay cell-cycle transitions at multiple points. The genetic control of these delays, termed `checkpoints\', was first established in budding yeast where it was shown that the RAD9 gene functions in G 2/M arrest after irradiation with X-rays. Subsequently, it has become clear that Rad9 also functions at the G 1/S, intra-S and mid-anaphase checkpoints. Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR). Rad9 may also have a pro-apoptotic function. This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kD. The human homolog is reported at 48.5kD.
Immunogen: Whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of aa1125-1139 of 1309 of yeast Rad9 protein (KLH coupled).