anti-RAF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; RAF1; anti-RAF1 antibody;
The RAF1 raf1 (Catalog #MBS624544) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAF1, phosphorylated (Ser338) (RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, Raf-1, C-RAF, cRaf, RAF) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RAF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA).Suitable for use in ELISA, Western Blot, and Dot Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:200
Dot Blot: 1:500. Researchers should empirically determine the suitability of the RAF1 raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAF1 raf1 product has the following accession number(s) (GI #496091) (Uniprot Accession #P04049). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAF1, phosphorylated (Ser338) (RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, Raf-1, C-RAF, cRaf, RAF) with the following immunoassay(s):
Dot Blot (DB) (Dot blot analysis of anti-RAF1-pS338 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB) (Western blot analysis of Phospho-RAF1-pS338 Antibody in human TPA activated Hela cell line lysates. Phospho-RAF1 (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min))
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser338 of human RAF1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RAF1 are readily searchable from our website. Different antibodies against the same target such as RAF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.