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anti-RAG2 antibody product blog

Posted on 2015-12-10 11:43:26 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RAG2; anti-RAG2 antibody;
The RAG2 rag2 (Catalog #MBS2516680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAG2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RAG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the RAG2 rag2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RAG2 rag2 product has the following accession number(s) (GI #45737860) (NCBI Accession #AAS75812.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAG2 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RAG2 antibody.)
Western Blot (WB) RAG2.

Immunofluorescence (IF) (Immunofluorescence analysis of U20S cell using RAG2 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) RAG2.

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Immunogen: Recombinant protein of human RAG2
Observed Molecular Weight: 59kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RAG2 are readily searchable from our website. Different antibodies against the same target such as RAG2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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