anti-RBC antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-RBC antibody; RBC;
The RBC rbcl (Catalog #MBS524315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM) reacts with Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s RBC can be used in a range of immunoassay formats including, but not limited to, This reagent is suitable for use in cytotoxic assays. Researchers should empirically determine the suitability of the RBC rbcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The RBC rbcl product has the following accession number(s) (GI #37087684) (NCBI Accession #O93627.5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
MBS524315 is rabbit IgM directed against Bovine red blood cells. This IgM fraction was isolated from a high titer antiserum by salt fractionation, ion exchange and gel filtration chromatography. It was tittered against its immunogens, sterile filtered and analyzed for immunoglobulin purity and protein concentration. This IgM fraction contains agglutinating and lysing antibodies and can be used for these procedures. .
Preservatives: 0.05% Sodium Azide (NaN3). Test Type: Hemmagglutaination:
Test Cell Suspension: 100 uL of washed 1% fixed bovine red blood cells in saline.
Product Tested: 100 ul of the above listed product and lot diluted in 1:100 heat inactivated normal rabbit serum in saline.
Incubation: 37�C for 20 mins, in U-bottom microtiter plates.
20�C for 2-4 hrs. for cell settling pattern.
Titer: The highest dilution of the product that yields agglutination.
Results: Titer of 1:100
Ouchterlony
Sample size: 10uL
Distance between cells 5mm
Test time : 72 hours.
Antiserum tested: Goat anti-rabbit IgM, goat anti-rabbit IgG . In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RBC are readily searchable from our website. Different antibodies against the same target such as RBC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.