anti-RBM14 antibody product blog
Tags: Antibody; Polyclonal Antibody; Rbm14; anti-RBM14 antibody;
The RBM14 rbm14 (Catalog #MBS9206830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBM14 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. MyBioSource\'s RBM14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
. Researchers should empirically determine the suitability of the RBM14 rbm14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RBM14 rbm14 product has the following accession number(s) (GI #311771525) (NCBI Accession #NP_001185765.1) (Uniprot Accession #Q96PK6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RBM14 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (RBM14 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM14 antibody detected the RBM14 protein (arrow).)
Immunohistochemistry (IHC) (RBM14 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM14 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (RBM14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN
Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
Cellular Location: Nucleus. Nucleus, nucleolus. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles
Tissue Location: Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RBM14 are readily searchable from our website. Different antibodies against the same target such as RBM14 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.