anti-RCVRN antibody product blog
Tags: Antibody; Polyclonal Antibody; Recoverin; RCVRN; anti-RCVRN antibody;
The RCVRN rcvrn (Catalog #MBS630737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recoverin, CT (Cancer-associated Retinopathy Protein, Protein CAR, RCVRN, RCV1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Recoverin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the RCVRN rcvrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RCVRN rcvrn product has the following accession number(s) (GI #4506459) (NCBI Accession #NP_002894.1) (Uniprot Accession #P35243). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Recoverin, CT (Cancer-associated Retinopathy Protein, Protein CAR, RCVRN, RCV1) with the following immunoassay(s):
Western Blot (WB) (The anti-Recoverin C-term Pab is used in Western blot to detect Recoverin in Y79 cell lysate.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.
Immunogen: Synthetic peptide selected from the C-terminal region of human Recoverin (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RCVRN are readily searchable from our website. Different antibodies against the same target such as RCVRN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.