anti-RECQL4 antibody product blog
Tags: Antibody; Polyclonal Antibody; RECQL4; anti-RECQL4 antibody; RecQ protein-like 4;
The RECQL4 recql4 (Catalog #MBS613594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RecQ protein-like 4 (ATP-dependent DNA helicase Q4, RecQ4, RECQ4, RTS) reacts with Human, Monkey, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RecQ protein-like 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the RECQL4 recql4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RECQL4 recql4 product has the following accession number(s) (GI #73808276) (Uniprot Accession #O94761). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RecQ protein-like 4 (ATP-dependent DNA helicase Q4, RecQ4, RECQ4, RTS) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HeLa, A431 and H3255 cells using MBS613594 RecQ4 Antibody.)
RECQL4 is a member of the RecQ family of DNA helicases that plays an important role in global genomic stability. There are five members of this family in humans, and mutations in three of these, BLM, WRN and RECQL4, give rise to disorders that are characterized by premature aging and a predisposition to cancer (1). Despite the presence of a helicase domain, no helicase activity has been reported for RECQL4. Rather, RECQL4 has an ATPase function that is stimulated by ssDNA, and a ssDNA annealing activity that is inhibited by RPA (2). RECQL4 has been reported to interact with ubiquitin ligases UBR1 and UBR2 (3). The role of RECQL4 in tumor suppression and the maintenance of genomic integrity has been attributed to it\'s activities associated with the regulation of DNA replication, and DNA recombination and repair (4-6). Mutations in the RECQL4 gene have been identified in a subset of patients with Rothmund-Thomson syndrome (RTS) a disorder characterized by growth deficiency, skin and skeletal abnormalities, and cancer predisposition. Two more autosomal recessive disorders have been associated with RECQL4 gene mutations: RAPADILINO, and BallerGerold syndromes (4).
Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human RecQ4. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RECQL4 are readily searchable from our website. Different antibodies against the same target such as RECQL4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.