|The RELA rela (Catalog #MBS857340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NF kappa B-p65 (Phospho-Ser536) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NF kappa B-p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500~1:1000, IHC: 1:50~1:100, IF:1:100~1:200. Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RELA rela product has the following accession number(s) (GI #223468681) (NCBI Accession #NP_001138610.1) (Uniprot Accession #Q04206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NF kappa B-p65 (Phospho-Ser536) Antibody with the following immunoassay(s):
Testing Data (P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFkappaB-p65 (phospho-Ser311) antibody (E011260).)
Testing Data #2 (Western blot analysis of extract from Hela cells untreated or treated with IFN, using NFkappaB-p65 (Ab-311) antibody and NFkappaB-p65 (phospho-Ser311) antibody (E011260, Lane 3 and 4).)
Testing Data #3 (P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (phospho-Thr254) antibody (E011010).)
Testing Data #4 (P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (phospho-Thr435) antibody (E011012).)
Testing Data #5 (Western blot analysis of extracts from COS7 cells using NF-kappaB p65 (Ab-435) antibody (E021012, Line 1 and 2) and NF-kappaB p65 (phospho-Thr435) antibody (E011012, Line 3 and 4).)
Testing Data #6 (Western blot analysis of extracts using NF-kappaB p65 (phospho-Ser468) antibody (E011013).)
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
Pathway: NF-kappa B, Akt, Cancer/Apoptosis, Chromatin/Transcription. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RELA are readily searchable from our website. Different antibodies against the same target such as RELA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. RELA also interacts with the following gene(s): CHUK, COMMD1, CREBBP, EP300, HDAC1, IKBKB, NFKB1, NFKBIA, NFKBIB, UBC. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Heart, Intestine, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein.