|The RELA rela (Catalog #MBS9201546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RELA Antibody (N-term) reacts with Human (Predicted Reactivity: Chicken, Mouse, Xenopus) and may cross-react with other species as described in the data sheet. MyBioSource\'s RELA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB).
WB~~1:1000. Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RELA rela product has the following accession number(s) (GI #223468681) (NCBI Accession #NP_001138610.1) (Uniprot Accession #Q04206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RELA Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (RELA Antibody (N-term) western blot analysis in Hela,MCF-7,Raji,Ramos cell line lysates (35ug/lane).This demonstrates the RELA antibody detected the RELA protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with RELA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (RELA Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of RELA Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an An or G purine; and Y is a C or T pyrimidine).
Antigen Type: Synthetic Peptide
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RELA are readily searchable from our website. Different antibodies against the same target such as RELA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.