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anti-RHEB antibody product blog

Posted on 2015-12-31 05:23:14 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RHEB; anti-RHEB antibody;
The RHEB rheb (Catalog #MBS9402309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHEB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RHEB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100. Researchers should empirically determine the suitability of the RHEB rheb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RHEB rheb product has the following accession number(s) (GI #5032041) (NCBI Accession #NP_005605.1) (Uniprot Accession #Q15382). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RHEB Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of brian cell and Hela cell lysate using RHEB antibody.)
Western Blot (WB) RHEB.

Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin\'s GAP activity (7).

Immunogen Type: Recombinant Protein
Immunogen Description: Recombinant protein of human RHEB. Target Name: RHEB. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RHEB are readily searchable from our website. Different antibodies against the same target such as RHEB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Embryonic Tissue, Eye, Kidney, Liver, Lung, Pancreas, Skin, Vascular tissues are correlated with this protein.
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