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anti-RNF168 antibody product blog

Posted on 2015-11-09 09:52:22 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RNF168; anti-RNF168 antibody;
The RNF168 rnf168 (Catalog #MBS129343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF168 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RNF168 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
WB: 1:1000 - 1:2000
IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the RNF168 rnf168 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RNF168 rnf168 product has the following accession number(s) (GI #74762499) (NCBI Accession #Q8IYW5.1) (Uniprot Accession #Q8IYW5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RNF168 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RNF168 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.)
Western Blot (WB) RNF168.

This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome.

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human RNF168
Calculated Molecular Weight: 65kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RNF168 are readily searchable from our website. Different antibodies against the same target such as RNF168 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. RNF168 also interacts with the following gene(s): H2AFX, OTUB1, SUMO1, TP53BP1, UBC, UBE2N. Breast Neoplasms, Cardiovascular Diseases, Embryo Loss, Learning Disorders, Mental Disorders, Neurobehavioral Manifestations, Riddle Syndrome, Vascular Diseases are some of the diseases may be linked to RNF168 Polyclonal Antibody. Bone, Brain, Kidney tissues are correlated with this protein.
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