anti-RNF25 antibody product blog
Tags: Antibody; Polyclonal Antibody; RNF25; anti-RNF25 antibody;
The RNF25 rnf25 (Catalog #MBS240518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human RNF25 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RNF25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA).ELISA (1:10000 - 1:40000), IHC-P (5 ug/ml), IP, WB (1:200 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the RNF25 rnf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RNF25 rnf25 product has the following accession number(s) (GI #34878787) (NCBI Accession #NP_071898.2) (Uniprot Accession #Q96BH1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human RNF25 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-RNF25 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (Anti-RNF25 Antibody - Western Blot. Western blot of affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.)
Western Blot (WB) (Anti-RNF25 Antibody - Immunoprecipitation. 1 ug of affinity purified anti-RNF25 was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.)
Target Species: Human
Immunogen Description: Synthetic peptide from human RNF25. Immunogen Type: Synthetic peptide
Immunogen: RNF25 antibody was raised against synthetic peptide from human RNF25.
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RNF25 are readily searchable from our website. Different antibodies against the same target such as RNF25 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.