anti-ROBO2 antibody product blog
Tags: Antibody; Polyclonal Antibody; ROBO2; anti-ROBO2 antibody;
The ROBO2 robo2 (Catalog #MBS9129501) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ROBO2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s ROBO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the ROBO2 robo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ROBO2 robo2 product has the following accession number(s) (GI #193083163) (NCBI Accession #NP_001122401.1) (Uniprot Accession #Q9HCK4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ROBO2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ROBO2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.)
The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants.
Source: Rabbit
Immunogen: Recombinant protein of human ROBO2. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ROBO2 are readily searchable from our website. Different antibodies against the same target such as ROBO2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Cholangiocarcinoma, Congenital Abnormalities, Disease Models, Animal, Inflammation, Liver Diseases, Liver Neoplasms, Lung Diseases, Nervous System Diseases, Urogenital Abnormalities, Vesicoureteral Reflux 2 are some of the diseases may be linked to ROBO2 Polyclonal Antibody. Brain, Embryonic Tissue, Eye, Heart, Lung, Muscle, Ovary, Pancreas, Placenta, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene.