anti-ROBO3 antibody product blog
Tags: Antibody; Polyclonal Antibody; Robo3; anti-ROBO3 antibody;
The ROBO3 robo3 (Catalog #MBS2527884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROBO3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s ROBO3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC).IHC: 1:25-1:100. Researchers should empirically determine the suitability of the ROBO3 robo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ROBO3 robo3 product has the following accession number(s) (GI #48476182) (NCBI Accession #NP_071765.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ROBO3 Polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using ROBO3 Polyclonal Antibody at dilution 1:35)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using ROBO3 Polyclonal Antibody at dilution 1:35)
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.
Immunogen: Recombinant protein of human ROBO3. Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ROBO3 are readily searchable from our website. Different antibodies against the same target such as ROBO3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.