anti-RORA antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-RORA antibody; RORA;
The RORA rora (Catalog #MBS9125881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RORA Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RORA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the RORA rora for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RORA rora product has the following accession number(s) (GI #613504304) (NCBI Accession #P35398.2) (Uniprot Accession #P35398). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RORA Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RORA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using RORA antibody. Blue: DAPI for nuclear staining.)
The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human RORA
Calculated Molecular Weight: 63kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RORA are readily searchable from our website. Different antibodies against the same target such as RORA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Embryonic Tissue, Eye, Kidney, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein.