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anti-RPL27 antibody product blog

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Posted on 2016-06-10 05:28:00 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; 60S ribosomal protein L27; anti-RPL27 antibody; RPL27;
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The RPL27 rpl27 (Catalog #MBS7005447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human 60S ribosomal protein L27 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s 60S ribosomal protein L27 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the RPL27 rpl27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RPL27 rpl27 product has the following accession number(s) (GI #4506623) (NCBI Accession #NP_000979.1) (Uniprot Accession #P61353). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human 60S ribosomal protein L27 polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: 60S ribosomal protein L27 antibody at 2ug/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
)
Western Blot (WB) RPL27.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human colorectal carcinoma using MBS7005447 at diluton of 1:50)
Immunohistochemistry (IHC) RPL27.

Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7005447 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) RPL27.

60S ribosomal protein L27 is a protein that in humans is encoded by the RPL27 gene.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen: Recombinant human 60S ribosomal protein L27 protein. Conjugation: Non-conjugated
Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-137716.
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