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anti-RPS6KA1 antibody product blog

Posted on 2016-01-05 02:32:12 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-RPS6KA1 antibody; RPS6KA1; RSK1;
The RPS6KA1 rps6ka1 (Catalog #MBS622629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rsk1, phosphorylated (Ser352) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, Mapkapk1a, MAPKAPK-1a, Ribosomal Prote reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Rsk1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500-1:1,000. Researchers should empirically determine the suitability of the RPS6KA1 rps6ka1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RPS6KA1 rps6ka1 product has the following accession number(s) (GI #55743134) (NCBI Accession #NP_001006666.1) (Uniprot Accession #Q15418). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylates threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators.

RPS6KA1 rps6ka1

Immunogen: Synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of serine 352 (R-D-SP-P-G). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RPS6KA1 are readily searchable from our website. Different antibodies against the same target such as RPS6KA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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