anti-RRM2B antibody product blog
Tags: Antibody; Polyclonal Antibody; p53R2; RRM2B; anti-RRM2B antibody;
The RRM2B rrm2b (Catalog #MBS176302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p53R2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s p53R2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RRM2B rrm2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The RRM2B rrm2b product has the following accession number(s) (GI #74727333) (NCBI Accession #Q7LG56.1) (Uniprot Accession #Q7LG56). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-p53R2 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-p53R2 antibody, MBS176302, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: HELA Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for Ribonucleoside-diphosphate reductase subunit M2 B(RRM2B) detection. Tested with WB in Human, Mouse, Rat.
Background: RRM2B(Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al.(2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al.(2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2 (318-332aa EGKTNFFEKRVSEYQ), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RRM2B are readily searchable from our website. Different antibodies against the same target such as RRM2B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.