|The RTCB rtcb (Catalog #MBS7051688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RTCB Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RTCB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the RTCB rtcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RTCB rtcb product has the following accession number(s) (GI #7657015) (NCBI Accession #NP_055121.1) (Uniprot Accession #Q9Y3I0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RTCB Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in:NIH/3T3 whole cell lysate,HEK293 whole cell lysate
All lanes: RTCB antibody at 3ug/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7051688 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7051688 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7051688 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3\', 5\'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Immunogen: Recombinant human tRNA-splicing ligase RtcB homolog protein (52-229AA). Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-86491 / sc-86492. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RTCB are readily searchable from our website. Different antibodies against the same target such as RTCB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. RTCB also interacts with the following gene(s): C14orf166, C22orf28, C2orf49, DDX1, FAM98B, RTCA, ZBTB8OS.