anti-RUNX2 antibody product blog
Tags: Antibody; RUNX2; Polyclonal Antibody; anti-RUNX2 antibody;
The RUNX2 runx2 (Catalog #MBS127554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUNX2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RUNX2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:200 - 1:1000
IHC: 1:20 - 1:50. Researchers should empirically determine the suitability of the RUNX2 runx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RUNX2 runx2 product has the following accession number(s) (GI #17368460) (NCBI Accession #Q13950.2) (Uniprot Accession #Q13950). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RUNX2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RUNX2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.
Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human RUNX2
Calculated Molecular Weight: 57kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RUNX2 are readily searchable from our website. Different antibodies against the same target such as RUNX2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Bone Marrow, Brain, Embryonic Tissue, Heart, Liver, Lung, Mouth, Muscle, Skin tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Cleidocranial Dysplasia, Craniofacial Abnormalities, Hypertrophy, Inflammation, Kidney Diseases, Necrosis, Neoplasm Invasiveness, Prostatic Neoplasms are some of the diseases may be linked to RUNX2 Polyclonal Antibody.